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  • prrs-awards-placeholder
    The 12th European PRRS Research Awards
  • combat
    COMBAT - Updated biosecurity tool with 4 features
  • Cover Guilty Gilt
    The Guilty Gilt Guide
  • PRRS Ctrl
    PRRS Ctrl 2.0
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Top publications | November

Disinfection and conditions associated with thermo-assisted drying and decontamination inconsistently produce negative PRRSV rRT-PCR results on metal surfaces

Because contaminated livestock trailers are a significant risk for transmitting viruses between herds, various methods of washing, disinfecting, and thermo-assisted drying and decontamination (TADD) have been evaluated for their effectiveness in inactivating porcine reproductive and respiratory syndrome virus (PRRSV) on contaminated surfaces. Information on when to expect negative qRT-PCR results after adequate trailer sanitation is lacking. The objective of this study was to evaluate whether there are conditions associated with washing-disinfectant-TADD procedures that will consistently produce a negative qRT-PCR result for the purpose of monitoring compliance with trailer sanitation and decontamination protocols for PRRSV on metal surfaces. 144 diamond plate aluminum coupons were spiked with PRRSV or phosphate-buffered saline (PBS) and treated with a designated disinfectant protocol. Disinfectants evaluated included multiple accelerated® hydrogen peroxide (AHP) disinfectants and a quaternary ammonium and glutaraldehyde combination disinfectant. Disinfectant was applied for 5 or 60 minutes of contact time at either 20 °C or −10 °C in a matrix of feces or PBS. All coupons were heated until the surface temperature of the coupon reached 71 °C and then held for 10 minutes to simulate TADD under field conditions. Post-treatment swabs for all treatment groups, except negative control groups, were positive by PRRSV qRT-PCR. Under the conditions evaluated in this study, consistently negative qRT-PCR results after treatments were not found. Therefore, for the purpose of monitoring compliance with trailer sanitation and decontamination protocols for PRRSV, alternatives to qRT-PCR should be explored.

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Expertise article

Experimental reproduction of severe disease in CD/CD pigs concurrently infected with type 2 porcine circovirus and porcine reproductive and respiratory syndrome virus

Three-week-old cesarean-derived colostrum-deprived (CD/CD) pigs were inoculated with porcine circovirus type 2 (PCV2, n 19), porcine reproductive and respiratory syndrome virus (PRRSV, n 13), concurrent PCV2 and PRRSV (PCV2/PRRSV, n 17), or a sham inoculum (n 12) to compare the independent and combined effects of these agents. Necropsies were performed at 7, 10, 14, 21, 35, and 49 days postinoculation (dpi) or when pigs became moribund. By 10 dpi, PCV2/PRRSV-inoculated pigs had severe dyspnea, lethargy, and occasional icterus; after 10 dpi, mortality in this group was 10/11 (91%), and all PCV2/ PRRSV-inoculated pigs were dead by 20 dpi. PCV2-inoculated pigs developed lethargy and sporadic icterus, and 8/19 (42%) developed exudative epidermitis; mortality was 5/19 (26%). PRRSV-inoculated pigs developed dyspnea and mild lethargy that resolved by 28 dpi. Microscopic lesions consistent with postweaning multisystemic wasting syndrome (PMWS) were present in both PCV2- and PCV2/PRRSV-inoculated pigs and included lymphoid depletion, necrotizing hepatitis, mild necrotizing bronchiolitis, and infiltrates of macrophages that occasionally contained basophilic intracytoplasmic inclusion bodies in lymphoid and other tissues. PCV2/ PRRSV-inoculated pigs also had severe proliferative interstitial pneumonia and more consistent hepatic lesions. The most severe lesions contained the greatest number of PCV2 antigen–containing cells. PRRSV-inoculated pigs had moderate proliferative interstitial pneumonia but did not develop bronchiolar or hepatic lesions or lymphoid depletion. All groups remained seronegative to porcine parvovirus. The results indicate that 1) PCV2 coinfection increases the severity of PRRSV-induced interstitial pneumonia in CD/CD pigs and 2) PCV2 but not PRRSV induces the lymphoid depletion, granulomatous inflammation, and necrotizing hepatitis characteristic of PMWS.

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Expertise article | Top publications | October

Economic losses of four porcine reproductive and respiratory syndrome outbreak cases by calculating increased costs and decreased revenues in China

The economic impact after the outbreak of porcine reproductive and respiratory syndrome (PRRS) has been proven to be tremendous for pig production worldwide. However, the economic impact of the disease is not well understood in China.

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Asian PRRSpective | AS 2018

African swine fever: can PRRS knowledge guide us?

12th Asian PRRSpective 2018 · Building together a strong base for PRRS control

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Episodes

Episode 13 - Preparing gilts for breeding: Development and acclimation

“It’s important that pathogen exposure occurs early enough in the gilt’s life so she recovers from the infection, stops shedding the pathogen and is immunocompetent at the time of farrowing”

2018 European PRRSpective

Practical diagnostic sampling for large scale monitoring

4th European PRRSpective. Building together a strong base for PRRS control

27th September, 2018

Impact on daily growth during multiple influenza coughing outbreaks
Expertise video

Impact on daily growth during multiple influenza coughing outbreaks

Cough is a common symptom of finishers. Nowadays, real-time technology is available for pig cough recognition as part of a precision livestock farming system1,2. However, once cough is recognized in the field, there is a need for a better understanding of its concurrent impact on growth performance. Therefore, the objective of this study was to compare and quantify the impact in daily growth (ADGW) of disease-coughing outbreaks occurring in a finishing batch of pigs under commercial conditions.

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Expertise article

General guidelines for porcine reproductive and respiratory syndrome regional control and elimination projects

Porcine reproductive and respiratory syndrome (PRRS) continues to be a costly disease affecting the swine industry world- wide.